Oral anti-inflammatory activity of cannabidiol, a
non-psychoactive constituent of cannabis, in acute carrageenan-induced
inflammation in the rat paw.
Costa B, Colleoni M, Conti S,
Parolaro D, Franke C, Trovato AE, Giagnoni G.
Naunyn Schmiedebergs Arch Pharmacol. 2004 Feb 12 [Epub ahead of print]
Biotecnologie e Bioscienze, Universita di Milano-Bicocca, Piazza della Scienza
2, 20126, Milan, Italy.
Cannabidiol, the major non-psychoactive component
of marijuana, has various pharmacological actions of clinical interest. It is
reportedly effective as an anti-inflammatory and anti-arthritic in murine
collagen-induced arthritis. The present study examined the anti-inflammatory and
anti-hyperalgesic effects of cannabidiol, administered orally (5-40 mg/kg) once
a day for 3 days after the onset of acute inflammation induced by intraplantar
injection of 0.1 ml carrageenan (1% w/v in saline) in the rat. At the end of the
treatment prostaglandin E2 (PGE2) was assayed in the plasma, and cyclooxygenase
(COX) activity, production of nitric oxide (NO; nitrite/nitrate content), and of
other oxygen-derived free radicals (malondialdehyde) in inflamed paw tissues.
All these markers were significantly increased following carrageenan.Thermal
hyperalgesia, induced by carrageenan and assessed by the plantar test, lasted 7
h. Cannabidiol had a time- and dose-dependent anti-hyperalgesic effect after a
single injection. Edema following carrageenan peaked at 3 h and lasted 72 h; a
single dose of cannabidiol reduced edema in a dose-dependent fashion and
subsequent daily doses caused further time- and dose-related reductions.There
were decreases in PGE2 plasma levels, tissue COX activity, production of
oxygen-derived free radicals, and NO after three doses of cannabidiol. The
effect on NO seemed to depend on a lower expression of the endothelial isoform
of NO synthase. In conclusion, oral cannabidiol has a beneficial action on two
symptoms of established inflammation: edema and hyperalgesia.
14963641 [PubMed - as supplied by publisher]
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